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The spatial positions of cells in the Pellioditis marina embryo are largely determined by the cell lineage. The movie shows a simulation of the embryonic development of P. marina based on 4D microscopy data (Houthoofd et al., 2003). Development runs for approximately 530 minutes from shortly after the third cell division (4-cell stage) until the onset of muscle contraction (571-cell stage). Initially, the embryo is viewed from the ventral side, with anterior to the left, and then rotates to a left lateral position during elongation. Cells are coloured in a gradient from red to blue corresponding to their position in the lineage diagram from left to right (Supplementary Fig. S3). Cells undergoing programmed cell death turn white and become increasingly transparent before vanishing.

P. marina movie, small
Small (Quicktime; 10 MB)
  P. marina movie, large
Large (Quicktime; 167.5 MB)